Histone H2A.X rabbit pAb

• Catalog Number: orb765400
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H2A.X
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H2A.X. AA range:94-143
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. IP 1:200-500,ELISA: 1/10000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 19
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-H2AFX antibody, anti-H2AX antibody, anti-Hist
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of Hela cell. 1, Histone H2A.X Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3B Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Immunofluorescence analysis of rat-lung tissue. 1, Histone H2A.X Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunofluorescence analysis of rat-spleen tissue. 1, Histone H2A.X Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Histone H2A.X Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.