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GRP78 Antibody: FITC

Catalog Number: orb181938

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SizePriceQuantity
100 μg$ 630.00
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Catalog Numberorb181938
CategoryAntibodies
DescriptionMouse monoclonal antibody against GRP78, conjugated to FITC.
TargetGRP78
ClonalityMonoclonal
Species/HostMouse
IsotypeIgG1 Kappa
ConjugationFITC
ReactivityHuman, Mouse, Rat
Concentration1 mg/ml
Buffer/Preservatives640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS
PurificationProtein G Purified
ImmunogenFull-length recombinant rat GRP78
UniProt IDP06761
MW78kDa
Tested applicationsICC, IF, IHC
Dilution rangeWB (1:1000), ICC/IF (1:100)
Application notes1 µg/ml of SMC-211 was sufficient for detection of GRP78 in 20 µg of HEK-293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Clone Number3G12-1G11
StorageConjugated antibodies should be stored according to the product label
Alternative names78 kDa glucose regulated protein antibody, 78 kDa
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Research AreaCancer Biology, Cell Biology, Protein Biochemistry
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NoteFor research use only
Entrez25617
NCBINP_037215.1
Expiration Date12 months from date of receipt.
GRP78 Antibody: FITC

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3G12-1G11. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.

GRP78 Antibody: FITC

Western Blot analysis of Human, Mouse, Rat HEK-293, NIH3T3, and Rat Brain cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3G12-1G11. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.

GRP78 Antibody: FITC

ELISA analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3G12-1G11. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody.

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