GRP78 Antibody (FITC)

SKU: orb151310

Description

Rabbit polyclonal to GRP78 (FITC). GRP78 is a ubiquitously expressed, 78-kDa glucose regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed. GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis. Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival. In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress, and its protein levels are reduced in the brains of Alzheimers patients. Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs ..

Research Area

Cell Biology

Images & Validation

• Tested Applications: ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Bovine, Canine, Frog, Fungi, Hamster, Human, Monkey, Mouse, Plant, Rabbit, Rat
• Application Notes:
  A 1:1000 dilution was sufficient for detection of Grp78 in 10 µg of rat tissue lysate by ECL immunoblot analysis.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Rat GRP78 (Bip) synthetic peptide conjugated to KLH
• Target: GRP78
• Molecular Weight: 78kDa
• Purification: Peptide Affinity Purified
• Conjugation: FITC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GRP78, GRP-78, Grp78, GRP 78, HSPA5, HSPA 5, Heat shock 70 kDa protein 5, Heat Shock 70kDa Protein 5, BiP, BIP, Binding immunoglobulin protein, Immunoglobulin heavy chain-binding protein, Immunoglobulin Heavy Chain Binding Protein, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Endoplasmic reticulum lumenal, Ca2+ binding protein grp78, Glucose Regulated Protein 78kDa, 78 kDa glucose-regulated protein, 78 kDa glucose regulated protein, HSCe70, MIF2, mBiP, Sez7, AL022860, AU019543, D2Wsu141e, D2Wsu17e, FLJ26106, GRP78_HUMAN

Quick Database Links

Gene Symbol

GRP78

UniProt

P06761

NCBI Gene ID

25617

RefSeq (Protein):NP_037215.1