Goat anti-Vimentin Antibody

• Catalog Number: orb233645
• Category: Antibodies
• Description: Goat polyclonal antibody to Vimentin
• Target: Vimentin
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QVINETSQHHDDLE
• MW: 53.7
• Tested applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:4000, WB: 0.1-2 μg/ml, IHC-P: 5 μg/ml
• Application notes: WB: Approx 55kDa band observed in lysates of cell line HeLa and Jurkat. Recommended concentration: 1-3 μg/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CTRCT30 antibody, anti Epididymis luminal pro
• Note: For research use only
• Entrez: 7431

orb233645 (2µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb233645 (0.1µg/ml) staining of Mouse (A) and (2µg/ml) Rat (B) Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using Vimentin antibody.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using Vimentin antibody.

Immunohistochemical staining of Human Kidney using Vimentin antibody.