TFE3 Antibody

• Catalog Number: orb18418
• Category: Antibodies
• Description: Goat polyclonal antibody to TFE3
• Target: TFE3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SHAAEPARDGVEAS
• RRID: AB_10750740
• MW: 61.5
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave 50 and 30kDa bands in Raji and Daudi cell lysates at 2ug of primary. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 63Kda according to NP_006512. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti TFE3 antibody, anti transcription factor bind
• Research Area: Cancer, Epigenetics, Immunology
• Note: For research use only
• Entrez: 7030

Western blot analysis of HEK293 lysate using TFE3 antibody