Transcription factor E3 / TFE3 antibody

SKU: orb2276368

Description

Transcription factor E3 / TFE3 antibody validated for immunohistochemistry on 76 different Normal Tissues

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Kidney: A moderate to strong TFE3 staining should be seen in a fraction of glomerular cells and an at least weak TFE3 staining should be seen in a subset of tubular and collecting duct cells. Negative Control: Colon: TFE3 staining should be absent in epithelial cells (some very faint staining can occasionally occur at the surface). Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-403R
• Immunogen: Recombinant human TFE3 fragment
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BHLHE33

Strong nuclear TFE3 positivity in a renal cell carcinoma with TFE3 fusion.

Strong TFE3 staining of epithelial cells in the corpus epididymis.

TFE3 staining is lacking in epithelial cells of the appendix.

Quick Database Links

UniProt

P19532