Transcription factor E3 / TFE3 antibody

SKU: orb2276368

Description

Transcription factor E3 / TFE3 antibody validated for immunohistochemistry on 76 different Normal Tissues

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Kidney: A moderate to strong TFE3 staining should be seen in a fraction of glomerular cells and an at least weak TFE3 staining should be seen in a subset of tubular and collecting duct cells. Negative Control: Colon: TFE3 staining should be absent in epithelial cells (some very faint staining can occasionally occur at the surface). Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

  Read more...

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-403R
• Immunogen: Recombinant human TFE3 fragment
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BHLHE33

Quick Database Links

UniProt

P19532