TFE3 Antibody (N-term)

SKU: orb1933263

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF - 1:10-50, WB - 1:2000
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD42485
• Immunogen: This TFE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TFE3. Antigen Region: 1-30 aa.
• Target: TFE3 {ECO:0000303|PubMed:9393982, ECO:0000312|HGNC:HGNC:11752}
• Molecular Weight: 61521 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Transcription factor E3, Class E basic helix-loop-helix protein 33, bHLHe33, TFE3, BHLHE33

Fluorescent confocal image of Hela cell stained with TFE3 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFE3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TFE3 immunoreactivity is localized to nucleus and Cytoplasm significantly.

TFE3 Antibody (N-term) western blot analysis in 293 cell line lysates (35 ug/lane). This demonstrates the TFE3 Antibody detected the TFE3 protein (arrow).

TFE3 Antibody (N-term) western blot analysis in mouse lung tissue lysates (35 ug/lane). This demonstrates the TFE3 antibody detected the TFE3 protein (arrow).

Anti-TFE3 Antibody (N-term) at 1:2000 dilution + HepG2 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 62 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

TFE3 {ECO:0000303|PubMed:9393982, ECO:0000312|HGNC:HGNC:11752}

UniProt

P19532

RefSeq (Protein):NP_006512.2