TFE3 Antibody (N-term)

• Catalog Number: orb1933263
• Category: Antibodies
• Description: TFE3 Antibody (N-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P19532
• MW: 61521 Da
• Tested applications: IF, WB
• Dilution range: WB - 1:1000, IF - 1:10-50
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Research Area: Immunology & Inflammation
• Note: For research use only

Fluorescent confocal image of Hela cell stained with TFE3 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFE3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TFE3 immunoreactivity is localized to nucleus and Cytoplasm significantly.

TFE3 Antibody (N-term) western blot analysis in 293 cell line lysates (35 ug/lane). This demonstrates the TFE3 Antibody detected the TFE3 protein (arrow).

TFE3 Antibody (N-term) western blot analysis in mouse lung tissue lysates (35 ug/lane). This demonstrates the TFE3 antibody detected the TFE3 protein (arrow).

Anti-TFE3 Antibody (N-term) at 1:2000 dilution + HepG2 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 62 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.