You have no items in your shopping cart.
Cart summary
Item 1 of 4
Item 1 of 4
TFE3 Antibody (N-term)
Catalog Number: orb1933263
| Catalog Number | orb1933263 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB20263 |
| Tested applications | IF, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Dilution range | IF: 1:10~50, WB: 1:2000, WB: 1:1000, WB: 1:1000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 61521 Da |
| Target | This TFE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TFE3. |
| UniProt ID | P19532 |
| NCBI | NP_006512.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Transcription factor E3, Class E basic helix-loop- Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Fluorescent confocal image of Hela cell stained with TFE3 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFE3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TFE3 immunoreactivity is localized to nucleus and Cytoplasm significantly.
TFE3 Antibody (N-term) western blot analysis in 293 cell line lysates (35 ug/lane). This demonstrates the TFE3 Antibody detected the TFE3 protein (arrow).
TFE3 Antibody (N-term) western blot analysis in mouse lung tissue lysates (35 ug/lane). This demonstrates the TFE3 antibody detected the TFE3 protein (arrow).
Anti-TFE3 Antibody (N-term) at 1:2000 dilution + HepG2 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 62 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
