SPHK1 Antibody

• Catalog Number: orb19997
• Category: Antibodies
• Description: Goat polyclonal antibody to SPHK1
• Target: SPHK1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DVDLESEKYRRLGE
• RRID: AB_10938640
• MW: 43.9; 51.1; 42.5
• Tested applications: ELISA, IP, WB
• Dilution range: ELISA: 1:16000, WB: 2-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2). Recommended concentration: 2-3 μg/ml.Experiment Notes: Immunoprecipitation: EB05266 has been used in nuclear lysates of HeLa cells and a band of ~45kDa band has been detected, see Igarashi N. et al. 2003: Figure 2B), PMID: 12954646.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SPHK1 antibody, anti sphingosine kinase 1 ant
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 8877

orb19997 (2ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.