SPHK1 Antibody

SKU: orb19997

Description

Goat polyclonal antibody to SPHK1

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:16000, WB: 2-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2). Recommended concentration: 2-3 μg/ml.Experiment Notes: Immunoprecipitation: EB05266 has been used in nuclear lysates of HeLa cells and a band of ~45kDa band has been detected, see Igarashi N. et al. 2003: Figure 2B), PMID: 12954646.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SPHK1
• Protein Sequence: DVDLESEKYRRLGE
• Molecular Weight: 43.9; 51.1; 42.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SPHK1 antibody, anti sphingosine kinase 1 antibody, anti SPHK antibody, anti SK1 antibody, anti SKase antibody

orb19997 (2ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

SPHK1

NCBI Gene ID

8877