SPHK1 Rabbit Polyclonal Antibody

• Catalog Number: orb7002
• Category: Antibodies
• Description: SPHK1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Human, Rabbit
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SPHK1 (1-100/384aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 42-52 kDa
• Target: SPHK1
• UniProt ID: Q9NYA1
• RRID: AB_10932752
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: sphingosine kinase 1; SK 1; SK1; SPHK 1; SPHK; SPK
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SPHK1) Polyclonal Antibody, Unconjugated (orb7002) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Heart (Mouse) Lysate at 30 ug, Primary: Anti-SPHK1 (orb7002) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 52kD.

Sample: Lane 1: Mouse Pancreas tissue lysates, Lane 2: Mouse Lung tissue lysates, Lane 3: Rat Kidney tissue lysates, Primary: Anti-SPHK1 (orb7002) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42-52 kDa, Observed band size: 50 kDa.