SPHK1 Antibody (N-term)

• Catalog Number: orb1929454
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB3619
• Tested applications: WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:500
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 42518 Da
• Target: This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SPHK1.
• UniProt ID: Q9NYA1
• NCBI: NP_001136074.1, NP_892010.2, NP_068807.2, NP_001136073.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Sphingosine kinase 1, SK 1, SPK 1, SPHK1, SPHK, SP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The anti-SphK1 Pab is used in Western blot (Lane 2) to detect c-myc-tagged SphK1 in transfected 293 cell lysate (a c-myc antibody is used as control in Lane 1).

Western blot analysis of anti-hSPHK1-M1 Pab in 293 cell line lysates transiently transfected with the SPHK1 gene (2 ug/lane). hSPHK1-M1 (arrow) was detected using the purified Pab.

Western blot analysis of SPHK1-M1 Antibody (arrow) using rabbit polyclonal SPHK1-M1 Antibody. 293T cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1-M1 gene. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody

Western blot analysis of lysate from A431 cell line, using SPHK1 Antibody (M1). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.