SIAH1 Rabbit Polyclonal Antibody

SKU: orb329760

Description

Rabbit polyclonal antibody to SIAH1

Research Area

Cell Biology, Protein Biochemistry, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 4

Tested Applications	IHC, WB
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Zebrafish

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the N terminal region of human SIAH1
Target	SIAH1
Molecular Weight	35kDa
Purification	Protein A purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	1.0 mg/ml
Disclaimer	For research use only

Alternative Names

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anti SIAH1A antibody

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Sample Tissue: Mouse Liver, Antibody Dilution: 1 ug/mL.

Rabbit Anti-SIAH1 antibody, Catalog Number: orb329760, Paraffin Embedded Tissue: Human Heart cell, Cellular Data: Epithelial cells of renal tubule, Antibody Concentration: 4.0-8.0 ug/mL, Magnification: 400X.

Application: Western blotting Species+tissue/cell type: Human embryonic kidney 293T How many ug’s of tissue/cell lysate run on the gel: 1: 50 ug human HEK-293T cell lysatePrimary antibody Dilution: 1:1000, Secondary antibody: Anti-rabbit-IgG Secondary antibody Dilution: 1:5000.

WB Suggested Anti-SIAH1 Antibody Titration: 1.25 ug/mL, ELISA Titer: 1:312500, Positive Control: Jurkat cell lysate.

Quick Database Links

Gene Symbol

SIAH1

UniProt

Q8IUQ4

RefSeq (Protein):NP_001006611

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001006611

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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