PPARGC1A Antibody

• Catalog Number: orb1821120
• Category: Antibodies
• Description: PPARGC1A Antibody
• Target: PPARGC1A
• Species/Host: Goat
• Conjugation: Unconjugated
• Predicted Reactivity: Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: RDSVSPPKSLFSQC
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

orb1821120 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb1821120 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb1821120 (3µg/ml) staining of A431 (A) and HepG2 (B) cell lysate, and (1.5ug/ml) HEK293 nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.