PPARGC1A Antibody

• Catalog Number: orb1239904
• Category: Antibodies
• Description: PPARGC1A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: PPARGC1A antibody was raised against a 17 amino acid peptide near the amino terminus of human PPARGC1A.The immunogen is located within amino acids 200 - 250 of PPARGC1A.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 91kDObserved: 100 kD
• Target: PPARGC1A
• UniProt ID: Q9UBK2
• NCBI: NP_037393
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PPARGC1A antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: PPARGC1A Antibody: LEM6, PGC1, PGC1A, PGC-1v, PPAR
• Note: For research use only
• Application notes: WB: 2 μg/mL; IHC: 1-2 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

WB Validation in Human Cell Lines. Loading: 15 µg of human cell lysate Antibodies: PPARGC1A orb1239904, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Human Lung. Loading: 15 µg of human lung lysate Antibodies: PPARGC1A orb1239904, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Mouse Skin. Loading: 15 µg of mouse skin lysate Antibodies: PPARGC1A orb1239904, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Rat Kidney. Loading: 15 µg of rat kidney lysate Antibodies: PPARGC1A orb1239904, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of PPARGC1A in MCF7 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling PPARGC1A with orb1239904 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of PPARGC1A in HELA Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PPARGC1A with orb1239904 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunohistochemistry Validation of PPARGC1A in Human Colon Carcinoma Tissue. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PPARGC1A antibody (orb1239904) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of PPARGC1A in Human Pancreas Tissue. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PPARGC1A antibody (orb1239904) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of PPARGC1A in Mouse Stomach Tissue. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PPARGC1A antibody (orb1239904) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of PPARGC1A in Rat Kidney Tissue. Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PPARGC1A antibody (orb1239904) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.