Goat anti-Pleckstrin Antibody

SKU: orb18824

Description

Goat polyclonal to Pleckstrin

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA: 1:16000, WB: 0.01-0.3 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 45kDa band observed in Human Heart and Peripheral Blood Mononucleocyte lysates (calculated MW of 40.1kDa according to NP_002655.2). Recommended for use at 0.05-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Pleckstrin
• Protein Sequence: EWIKAIQMASRTGK
• Molecular Weight: 40.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PLEK antibody, anti pleckstrin antibody, anti P47 antibody, anti p47 antibody, anti FLJ27168 antibody

orb18824 staining (0.05ug/ml) of Human PBMC lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Quick Database Links

Gene Symbol

Pleckstrin

NCBI Gene ID

5341