Goat anti-PHLDA2 / IPL Antibody

• Catalog Number: orb18616
• Category: Antibodies
• Description: Goat polyclonal antibody to PHLDA2
• Target: PHLDA2 / IPL
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EPSRPSPQPKPRTP
• RRID: AB_10751078
• MW: 17.1
• Tested applications: ELISA, FC, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 1 μg/ml, IHC-P: 3.75 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave no signal but low background in Jurkat and human placenta extracts at up to 1 μg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PHLDA2 antibody, anti pleckstrin homology-lik
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 7262
• Expiration Date: 12 months from date of receipt.

3.75 µg/ml staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH6, AP-staining.

3.75 µg/ml staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH6, AP-staining.

Primary incubation 1 hour at room temperature. Image A: MDA-MB-231 cell lysate at primary Ab concentration 2 µg/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.