PHLDA3/TIH1 Antibody

• Catalog Number: orb18884
• Category: Antibodies
• Description: Goat polyclonal antibody to PHLDA3
• Target: PHLDA3 / TIH1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: RARQSLGTGTLVS
• RRID: AB_10751386
• MW: 13.9
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 0.2-1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 70kDa band in Human Placenta lysate after 1 μg/ml antibody staining. This band was successfully blocked by the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 13.9kDa according to NP_036528. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PHLDA3 antibody, anti TIH1 antibody, anti ple
• Research Area: Apoptotic, Cancer, Neuroscience, Signaling Pathway
• Note: For research use only
• Entrez: 23612

Western blot analysis of HEK293 lysate using PHLDA3 antibody