PHLDA3/TIH1 Antibody

SKU: orb18884

Description

Goat polyclonal antibody to PHLDA3

Research Area

Apoptotic, Cancer, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:16000, WB: 0.2-1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 70kDa band in Human Placenta lysate after 1 μg/ml antibody staining. This band was successfully blocked by the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 13.9kDa according to NP_036528. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PHLDA3 / TIH1
• Protein Sequence: RARQSLGTGTLVS
• Molecular Weight: 13.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PHLDA3 antibody, anti TIH1 antibody, anti pleckstrin homology-like domain, family A, member 3 antibody, anti pleckstrin homology-like domain, family A, member 2 antibody, anti OTTHUMP00000033920 antibody

Western blot analysis of HEK293 lysate using PHLDA3 antibody

Quick Database Links

Gene Symbol

PHLDA3 / TIH1

NCBI Gene ID

23612