PIAS2 Antibody

SKU: orb19487

Description

Goat polyclonal antibody to PIAS2

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:8000, WB: 0.5 μg/ml, IHC-P: 3-5µg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Testis shows nuclear and extra-nuclear staining in spermatids. Recommended concentration, 3-5ug/ml.WB: Preliminary experiments gave an approx 90kDa band in lysates of cell line A431after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 68.2kDa according to NP_004662.2. The 90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PIAS2
• Protein Sequence: KEAMKVSSQPCTKIE
• Molecular Weight: 63.4; 68.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PIAS2 antibody, anti protein inhibitor of activated STAT, 2 antibody, anti MGC102682 antibody, anti MIZ1 antibody, anti PIASX-ALPHA antibody, anti PIASX-BETA antibody, anti SIZ2 antibody, anti miz antibody, anti Msx-interacting-zinc finger antibody, anti protein inhibitor of activated STAT X antibody, anti ARIP3 antibody

Immunohistochemical staining of Human Testis using PIAS2 antibody

Quick Database Links

Gene Symbol

PIAS2

NCBI Gene ID

9063