PIASx rabbit pAb

• Catalog Number: orb766080
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PIASx
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human PIAS2. AA range:10-59
• MW: 68
• Tested applications: ELISA, WB
• Dilution range: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-PIAS2 antibody, anti-PIASX antibody, anti-E3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using PIASx Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Western Blot analysis of COS7 cells using PIASx Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Western blot analysis of lysates from COS7 cells, using PIAS2 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).