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Lime1 (mouse) Antibody

SKU: orb18807

Description

Goat polyclonal to Lime1

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:128000, WB: 0.5-2 μg/ml
ReactivityMouse
Predicted ReactivityRat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 32kDa band observed in Mouse Heart lysates (predicted MW of 31kDa according to NP_076173.1). Recommended for use at 0.5-2 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetLime1 (mouse)
Protein SequenceENVYESIKEMGL
Molecular Weight29.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti Lime1 antibody, anti Lck interacting transmembrane adaptor 1 antibody, anti 2810038K19Rik antibody, anti LIME antibody, anti RIKEN cDNA 2810038K19 gene antibody, anti RP23-60M5.4 antibody, anti OTTMUSP00000018005 antibody

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Lime1 (mouse) Antibody

orb18807 staining (1ug/ml) of Mouse Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Lime1 (mouse) Antibody (orb18807)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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