Goat anti-Lime1 (mouse) Antibody

• Catalog Number: orb18807
• Category: Antibodies
• Description: Goat polyclonal to Lime1
• Target: Lime1 (mouse)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: ENVYESIKEMGL
• RRID: AB_10755592
• MW: 29.6
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:128000, WB: 0.5-2 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 32kDa band observed in Mouse Heart lysates (predicted MW of 31kDa according to NP_076173.1). Recommended for use at 0.5-2 μg/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti Lime1 antibody, anti Lck interacting transmem
• Note: For research use only

orb18807 staining (1ug/ml) of Mouse Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.