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LIME1 Antibody
SKU: orb43903
Description
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| Tested Applications | FC, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a |
| Clone No. | LIME-10 |
| Immunogen | COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin. |
| Target | LIME1 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−LITA1
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Anti-Hu LIME1 purified antibody (clone LIME-10) specificity verification by flow cytometry. Analysis of the antibody staining was performed on fixed and permeabilized cell suspension. Anti-Hu LIME1 purified antibody (clone LIME-10) (concentration in sample 1 µg/ml, red-filled histogram) binds specifically to intacellular LIME1 in transfected cells (upper panel), but not to Jurkat cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control purified antibody (MOPC-21) under same conditions (concentration in sample 1 µg/ml, black-dashed histogram).
Western blotting analysis of human LIME using mouse monoclonal antibody LIME-10 on lysates of J77-LIME transfectants and Jurkat cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 1 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. LIME was detected around 30 kDa.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
LIME1 Antibody (orb43903)
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