Anti-LIME1 Purified

• Catalog Number: orb43901
• Category: Antibodies
• Description: Mouse Monoclonal to LIME.
• Target: LIME1
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purity: Purified by protein-A affinity chromatography.
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.
• UniProt ID: Q9H400
• RRID: AB_10993266
• Tested applications: FC, IP
• Application notes: Flow cytometry: Recommended dilution: 8-12 μg/ml. Intracellular staining.
• Antibody Type: Primary Antibody
• Clone Number: LIME-06
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-LIME antibody
• Note: For research use only
• Entrez: 54923

Induction of LIME tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92; cat. no. orb44416) or anti-human CD4 (MEM-16; cat. no. orb44114), and LIME was immunoprecipitated from laurylmaltoside lysates with the LIME-06 antibody (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom).

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-LIME1 (LIME-06) purified antibody (concentration in sample 9 µg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 µl reagent / 100 µl of peripheral whole blood).

Separation of human CD3 positive LIME1 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-LIME1 (LIME-06) purified antibody (concentration in sample 9 µg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-LIME1 (LIME-06) purified antibody (concentration in sample 9 µg/ml, GAM APC).