ITM2B Antibody

SKU: orb20142

Description

Goat polyclonal antibody to ITM2B

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 0.1-0.3 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 33kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 30.3kDa according to NP_068839.1). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ITM2B
• Molecular Weight: 30.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti ITM2B antibody, anti integral membrane protein 2B antibody, anti ABRI antibody, anti BRI antibody, anti BRI2 antibody, anti BRICD2B antibody, anti E25B antibody, anti E3-16 antibody, anti FBD antibody, anti BRICHOS domain containing 2B antibody, anti OTTHUMP00000018394 antibody

orb20142 (1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

WB analysis of HepG2 cell lysate using ITM2B antibody

Immunohistochemical staining of Human Small Intestine using ITM2B antibody

Immunofluorescence analysis of A431 cells using ITM2B antibody

Immunofluorescence analysis of HeLa cells using ITM2B antibody

Flow cytometric analysis of HeLa cells using ITM2B antibody

Quick Database Links

Gene Symbol

ITM2B

NCBI Gene ID

9445