ITM2B Antibody

• Catalog Number: orb1249554
• Category: Antibodies
• Description: ITM2B Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Predicted Reactivity: Bovine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-HDKETYKLQRRETIK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 30-35kDa band observed in HepG2 cell lysates (calculated MW of 30.3kDa according to NP_068839.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat and Mouse Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 2.5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of A431 and HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ITM2B
• UniProt ID: Q9Y287
• NCBI: NP_068839.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ITM2B, integral membrane protein 2B, ABRI, BRI, BR
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 30-35kDa band observed in HepG2 cell lysates (calculated MW of 30.3kDa according to NP_068839.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat and Mouse Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 2.5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of A431 and HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249554 (0.3 ug/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249554 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Golgi apparatus and cytoplasmic staining.

orb1249554 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Golgi apparatus and cytoplasmic staining.

orb1249554 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

orb1249554 (2.5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.