ABRI/ITM2B Antibody

• Catalog Number: orb1538799
• Category: Antibodies
• Description: ABRI/ITM2B Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Reactivity: Bovine, Equine, Human, Monkey
• Immunogen: Peptide with sequence C-HDKETYKLQRRETIK, from the internal region of the protein sequence according to NP_068839.1.
• Concentration: 0.5 mg/ml
• Dilution range: IF (10 µg/ml), IHC, IHC-P (2.5 - 3.75 µg/ml), Peptide-ELISA (1:8000), WB (0.1 - 0.3 µg/ml)
• Conjugation: Unconjugated
• Target: ABRI / ITM2B
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Alternative names: ITM2B, ABri/ADan amyloid peptide, ABRI, BRI, BRI2,
• Note: For research use only
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx. 33kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 30.3kDa according to NP_068839.1). Recommended concentration: 1-3 ug/ml.
• Expiration Date: 12 months from date of receipt.

ABRI / ITM2B antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

ABRI / ITM2B antibody (0.3 µg/ml) staining of HepG2 cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

ABRI / ITM2B antibody flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton.