MAP4K1/HPK1 Antibody

SKU: orb669235

Description

Anti-MAP4K1/HPK1 Antibody. Tested in WB applications. This antibody reacts with Rat.

Images & Validation

• Tested Applications: WB
• Reactivity: Rat
• Application Notes:
  Western blot, 0.25-0.5μg/ml, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of rat MAP4K1/HPK1, which shares 88.2% and 100% amino acid (aa) sequence identity with human and mouse MAP4K1/HPK1, respectively.
• Target: Chromaffin granule amine transporter
• Molecular Weight: 97 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Disclaimer: For research use only

Alternative Names

Fascin-2; Retinal fascin; FSCN2

Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 97 KD. The expected band size for MAP4K1/HPK1 is at 97 KD.

Quick Database Links

Gene Symbol

Chromaffin granule amine transporter

UniProt

D3Z8I4