Goat anti-Hdac2 (mouse) Antibody

• Catalog Number: orb420062
• Category: Antibodies
• Description: Goat polyclonal antibody to Hdac2
• Target: Hdac2 (mouse)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: PEDAVHEDSGDE
• MW: 55.3
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:2000, WB: 1-2 μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Application notes: WB: Approx 55-60kDa band observed in nuclear lysates of cell line HEK293 (calculated MW of 55.3kDa according to NP_032255.2). Recommended concentration: 1-2 μg/ml. Primary incubation 1 hour at room temperature.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Hdac2; histone deacetylase 2; D10Wsu179e; YAF1; Yy
• Note: For research use only
• Entrez: 3066

Western blot analysis of HEK293 nuclear cell lysate using Hdac2 antibody.

Flow cytometric analysis of HeLa cells using Hdac2 antibody.

Immunofluorescence analysis of NIH3T3 cells using Hdac2 antibody.

Immunofluorescence analysis of U251 cells using Hdac2 antibody.