Hdac2 (mouse) Antibody

SKU: orb420062

Description

Goat polyclonal antibody to Hdac2

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:2000, WB: 1-2 μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Application Notes:
  WB: Approx 55-60kDa band observed in nuclear lysates of cell line HEK293 (calculated MW of 55.3kDa according to NP_032255.2). Recommended concentration: 1-2 μg/ml. Primary incubation 1 hour at room temperature.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Hdac2 (mouse)
• Protein Sequence: PEDAVHEDSGDE
• Molecular Weight: 55.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Hdac2; histone deacetylase 2; D10Wsu179e; YAF1; Yy1bp; mRPD3; HD2; OTTMUSP00000022803; YY1 transcription factor-binding protein

Western blot analysis of HEK293 nuclear cell lysate using Hdac2 antibody.

Flow cytometric analysis of HeLa cells using Hdac2 antibody.

Immunofluorescence analysis of NIH3T3 cells using Hdac2 antibody.

Immunofluorescence analysis of U251 cells using Hdac2 antibody.

Quick Database Links

Gene Symbol

Hdac2 (mouse)

NCBI Gene ID

3066