HDAC2 Rabbit Polyclonal Antibody

SKU: orb574293

Description

Rabbit polyclonal antibody to HDAC2

Images & Validation

• Tested Applications: ChIP, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HDAC2
• Target: HDAC2
• Molecular Weight: 65kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

HD2, RPD3, YAF1, KDAC2

Chromatin Immunoprecipitation (ChIP) Using HDAC2 antibody - middle region (orb574293) and HCT116 Cells.

Sample Type: Human Adult Placenta, Antibody dilution: 1.0 ug/ml.

Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

Rabbit Anti-HDAC2 antibody, Catalog Number: orb574293, Paraffin Embedded Tissue: Human Lung cell, Cellular Data: Epithelial cells of renal tubule, Antibody Concentration: 4.0-8.0 ug/ml, Magnification: 400X.

WB Suggested Anti-HDAC2 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: 293T cell lysate, HDAC2 is supported by BioGPS gene expression data to be expressed in HEK293T.

Quick Database Links

Gene Symbol

HDAC2

UniProt

Q92769

RefSeq (Protein):NP_001518