HDAC2 Rabbit Polyclonal Antibody

• Catalog Number: orb574293
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HDAC2
• Target: HDAC2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Purification: Affinity Purified
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HDAC2
• Protein Sequence: Synthetic peptide located within the following region: HKKGAKKARIEEDKKETEDKKTDVKEEDKSKDNSGEKTDTKGTKSEQLSN
• UniProt ID: Q92769
• MW: 65kDa
• Tested applications: ChIP, IHC, WB
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HD2, RPD3, YAF1, KDAC2
• Research Area: Cell Biology, Epigenetics & Chromatin, Protein Bio
• Note: For research use only
• NCBI: NP_001518
• Expiration Date: 12 months from date of receipt.

Chromatin Immunoprecipitation (ChIP) Using HDAC2 antibody - middle region (orb574293) and HCT116 Cells.

Sample Type: Human Adult Placenta, Antibody dilution: 1.0 ug/ml.

Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

Rabbit Anti-HDAC2 antibody, Catalog Number: orb574293, Paraffin Embedded Tissue: Human Lung cell, Cellular Data: Epithelial cells of renal tubule, Antibody Concentration: 4.0-8.0 ug/ml, Magnification: 400X.

WB Suggested Anti-HDAC2 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: 293T cell lysate, HDAC2 is supported by BioGPS gene expression data to be expressed in HEK293T.