HDAC2 Rabbit Polyclonal Antibody

SKU: orb573969

Description

Rabbit polyclonal antibody to HDAC2

Research Area

Epigenetics & Chromatin, Stem Cell & Developmental Biology

Images & Validation

−

Item 1 of 5

Tested Applications	ChIP, IHC-P, WB
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat

Related Conjugates & Formulations

−

Biotin

FITC

HRP

Key Properties

−

Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the middle region of human HDAC2
Target	HDAC2
Molecular Weight	65kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

−

HD2, RPD3, YAF1, KDAC2

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Chromatin Immunoprecipitation (ChIP) Using HDAC2 antibody - middle region (orb573969) and HCT116 Cells.

HDAC2 was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with orb573969 with 1:200 dilution. Western blot was performed using orb573969 at 1/1000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: HDAC2 IP with orb573969 in HEK293 Whole Cell Lysate. Lane 3: Input of HEK293 Whole Cell Lysate.

IHC Suggested Anti-HDAC2 antibody, Titration: 5 ug/ml, Positive Control: Breast.

Rabbit Anti-HDAC2 Antibody, Catalog Number: orb573969, Formalin Fixed Paraffin Embedded Tissue: Human Testis Tissue, Observed Staining: Nucleus, Cytoplasm, Primary Antibody Concentration: 1:600, Other Working Concentrations: 1:600, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

WB Suggested Anti-HDAC2 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:1562500, Positive Control: HT1080 cell lysate.

Quick Database Links

Gene Symbol

HDAC2

UniProt

Q92769

RefSeq (Protein):NP_001518

UniProt Details

−

No UniProt data available

NCBI Reference Sequences

−

Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001518

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

ChIP

Chromatin Immunoprecipitation

View Protocol

Find More Protocols