HDAC2 Rabbit Polyclonal Antibody

• Catalog Number: orb573969
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HDAC2
• Target: HDAC2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Purification: Affinity Purified
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HDAC2
• Protein Sequence: Synthetic peptide located within the following region: HKKGAKKARIEEDKKETEDKKTDVKEEDKSKDNSGEKTDTKGTKSEQLSN
• UniProt ID: Q92769
• MW: 65kDa
• Tested applications: ChIP, IHC-P, WB
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HD2, RPD3, YAF1, KDAC2
• Research Area: Epigenetics & Chromatin, Stem Cell & Developmental
• Note: For research use only
• NCBI: NP_001518
• Expiration Date: 12 months from date of receipt.

Chromatin Immunoprecipitation (ChIP) Using HDAC2 antibody - middle region (orb573969) and HCT116 Cells.

HDAC2 was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with orb573969 with 1:200 dilution. Western blot was performed using orb573969 at 1/1000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: HDAC2 IP with orb573969 in HEK293 Whole Cell Lysate. Lane 3: Input of HEK293 Whole Cell Lysate.

IHC Suggested Anti-HDAC2 antibody, Titration: 5 ug/ml, Positive Control: Breast.

Rabbit Anti-HDAC2 Antibody, Catalog Number: orb573969, Formalin Fixed Paraffin Embedded Tissue: Human Testis Tissue, Observed Staining: Nucleus, Cytoplasm, Primary Antibody Concentration: 1:600, Other Working Concentrations: 1:600, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

WB Suggested Anti-HDAC2 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:1562500, Positive Control: HT1080 cell lysate.