GABRB3 Antibody

SKU: orb20106

Description

Goat polyclonal antibody to GABRB3

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:8000, WB: 1 μg/ml, IHC-P: 2-4µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Cortex shows patchy staining near or at the neuronal cell membrane.. Recommended concentration, 2-4ug/ml.WB: Preliminary experiments in Human Brain (Cerebral Cortex, Frontal Cortex and Cerebellum) lysates gave no specific signal but low background (at antibody concentration up to 1 μg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GABRB3
• Protein Sequence: EKTAKAKNDRSK
• Molecular Weight: 54.1; 54.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti GABRB3 antibody, anti gamma-aminobutyric acid (GABA) A receptor, beta 3 antibody, anti MGC9051 antibody, anti GABA(A) receptor beta-3 subunit antibody, anti GABA-A receptor beta-3 subunit antibody, anti GABA-alpha receptor beta-2 subunit antibody, anti GABAA receptor beta-3 subunit antibody

orb20106 (2.5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

GABRB3

NCBI Gene ID

2562