GABAA Receptor ß3 Antibody

SKU: orb613674

Description

Our Anti-GABAA Receptor ß3 rabbit polyclonal primary antibody from Biorbyt is produced in-house. It detects mouse and rat GABAA Receptor ß3 and is antigen affinity purified from pooled serum.

Research Area

Disease Biomarkers, Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB: 0.736111111, IHC: 0.25
• Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Fusion protein from the cytoplasmic loop of the beta 3 subunit of rat GABAA receptor.
• Target: GABAA Receptor ß3
• Molecular Weight: 53
• Purification: Antigen Affinity Purified from Pooled Serum
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml rAlbumin and 50% glycerol.
• Concentration: Lot Dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ECA5 antibody, GABA alpha receptor beta-2 subunit antibody, GABA(A) receptor subunit beta-3 antibody, GABAA receptor beta 3 subunit antibody, GABAA receptor subunit beta 3 antibody, GABR B3 antibody, Gabrb3 antibody, Gamma aminobutyric acid (GABA) A receptor beta 3 antibody, Gamma aminobutyric acid receptor subunit beta 3 antibody, Gamma-aminobutyric acid receptor subunit beta-3 antibody, GBRB3_HUMAN antibody, MGC9051 antibody

Immunoblots showing GABAA Receptor α2 (Cat. orb319526) and GABAA Receptor ß3 (Cat. orb613674) subunit expression in the cytosolic (S2), extrasynaptic (non-PSD), and synaptic (PSD) fractions of untreated, bicuculline-pretreated (20μM, 1h), or netrin-1 treated (250ng/ml, 1h) rat hippocampal neuronal cultures.

Immunofluorescence of mouse retina showing staining of GABAA-R, β3-subunit (Cat. : orb613674, green, 1:300) and calbindin (red). The blue is DAPI staining nuclear DNA.

Western blot of rat brain lysate showing specific immunolabeling of the ~53 kDa β3-subunit of the GABAA-R.

Western blot of rat cortical neurons showing specific immunolabeling of the ~53 kDa β3-subunit of the GABAA-R (1:1000).

Quick Database Links

Gene Symbol

GABAA Receptor ß3

UniProt

P63079