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Catalog Number | orb19131 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to FZD8 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, EM, IF, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 3 μg/ml |
Conjugation | Unconjugated |
MW | 73.3 |
Target | FZD8 / frizzled 8 |
Entrez | 8325 |
Protein Sequence | SYPKQMPLSQV |
RRID | AB_10755760 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti FZD8 antibody, anti frizzled homolog 8 (Droso Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas, shows granular cytoplasmic staining of acinar cells. Cells of the Islets of Langerhans are weakly stained, if at all. Recommended concentration, 3ug/ml.WB: Approx 75kDa band observed in human heart and human pancreas lysates (calculated MW of 73.3kDa according to NP_114072.1). Recommended concentration: 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of Human Pancreas using FZD8 antibody.
orb19131 (3ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-