FZD8/frizzled 8 Antibody

SKU: orb19131

Description

Goat polyclonal antibody to FZD8

Research Area

Developmental Biology, Epigenetics, GPCR, Neuroscience, Signaling Pathways, Wnt

Images & Validation

• Tested Applications: ELISA, EM, IF, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas, shows granular cytoplasmic staining of acinar cells. Cells of the Islets of Langerhans are weakly stained, if at all. Recommended concentration, 3ug/ml.WB: Approx 75kDa band observed in human heart and human pancreas lysates (calculated MW of 73.3kDa according to NP_114072.1). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: FZD8 / frizzled 8
• Protein Sequence: SYPKQMPLSQV
• Molecular Weight: 73.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti FZD8 antibody, anti frizzled homolog 8 (Drosophila) antibody, anti HGNC:4046 antibody, anti FZ-8 antibody, anti hFZ8 antibody, anti frizzled (Drosophila) homolog 8 antibody, anti frizzled 8 antibody

Immunohistochemical staining of Human Pancreas using FZD8 antibody.

orb19131 (3ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-

Quick Database Links

Gene Symbol

FZD8 / frizzled 8

NCBI Gene ID

8325