Frizzled 8 Antibody

• Catalog Number: orb1247476
• Category: Antibodies
• Description: Frizzled 8 Antibody
• Target: FZD8
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-SYPKQMPLSQV
• UniProt ID: Q9H461
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in Human Pancreas lysates (calculated MW of 73.3kDa according to NP_114072.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Pancreas. Recommended concentration: 3ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FZD8, frizzled homolog 8 (Drosophila) , HGNC:4046,
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_114072.1

orb1247476 (1 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247476 (3 ug/ml) staining of paraffin embedded Human Pancreas.