Anti-Frizzled 5 Antibody

• Catalog Number: orb665816
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Frizzled 5
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Frizzled 5. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-1000, IF: 1-100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: FZD5; FZD8
• Entrez: 14367, 14370, 317674, 7855, 8325, 100909849
• UniProt ID: Q9H461, Q8CHL0, Q498S8, Q9EQD0, Q13467, Q61091
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-FZD5 antibody, anti-C2orf31 antibody, anti-Fr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Frizzled 5 expression in SGC7901 (A), PC3 (B), MEF (C) whole cell lysates. (Predicted band size: 64; 73 kD; Observed band size: 65 kD)

Immunofluorescent analysis of Frizzled 5 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.