ERBB2/HER2 Antibody

• Catalog Number: orb233622
• Category: Antibodies
• Description: Goat polyclonal antibody to HER2
• Target: ERBB2 / HER2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: PREYVNARHCLP
• MW: 138; 135
• Tested applications: ELISA, IF, IHC
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000. IHC: In paraffin embedded Human breast cancer shows cell membrane and cytoplasm staining in the affected cells of Her+ type and not in triple negative type. Recommended concentration: 4-6µg/ml. Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-CD340 antibody, anti- ERBB2 antibody, anti- H
• Note: For research use only
• Entrez: 2064
• Expiration Date: 12 months from date of receipt.

4 µg/ml staining of paraffin embedded Human breast cancer (Her+ left, triple negative right). Steamed antigen retrieval with citrate buffer pH6, HRP-staining.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic and PM/cell projection staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.