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ERBB2/HER2 Antibody

SKU: orb233622

Description

Goat polyclonal antibody to HER2

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA, IF, IHC
Dilution RangePeptide ELISA: antibody detection limit dilution 1:128000. IHC: In paraffin embedded Human breast cancer shows cell membrane and cytoplasm staining in the affected cells of Her+ type and not in triple negative type. Recommended concentration: 4-6µg/ml. Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml.
ReactivityHuman

Key Properties

ClonalityPolyclonal
TargetERBB2 / HER2
Protein SequencePREYVNARHCLP
Molecular Weight138; 135
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti-CD340 antibody, anti- ERBB2 antibody, anti- HER 2 antibody, anti- HER 2/neu antibody, anti- HER2 antibody, anti- MLN 19 antibody, anti- MLN19 antibody, anti- NEU antibody, anti- NGL antibody, anti- p185 antibody, anti- p185erbB2 antibody, anti- Proto oncogene c ErbB 2 antibody, anti- Proto oncogene Neu antibody, anti- TKR1 antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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