Phospho-ERBB2(Y1127) Antibody

• Catalog Number: orb1930996
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This ERBB2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1127 of human ERBB2.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P04626
• MW: 137910 Da
• Tested applications: DOT, IF, WB
• Dilution range: IF: 1:100, WB: 1:5000, WB: 1:1000, DB: 1:500
• Clone Number: RB40080
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Receptor tyrosine-protein kinase erbB-2, Metastati
• Note: For research use only
• NCBI: NP_004439.2, NP_001005862.1

Dot blot analysis of Phospho-ERBB2-Y1127 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Fluorescent confocal image of MCF7 cells stained with phospho-ERBB2-Y1127 antibody. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with phospho-ERBB2- Y1127 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

Western blot analysis of extracts from A431 cells, untreated or treated with EGF, 100ng/ml, using Phospho-ERBB2-Y1127 or ERBB2 antibody (right).

Western blot analysis of extracts from A431 cells, untreated or treated with EGF, 100ng/ml, using phospho ERBB2-Y1127 (left) or ERBB2 antibody (right)