CHFR Antibody

• Catalog Number: orb18717
• Category: Antibodies
• Description: Goat polyclonal antibody to CHFR
• Target: CHFR
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KFNHICEQTRFKN
• RRID: AB_10754039
• MW: 73.4; 73.3; 72.0; 69.2; 63.9
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:64000, WB: 0.5ug/ml, IHC-P: 2.5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded human brain (cerebellum) shows stained Purkinje cells. Recommended concentration, 2.5ug/ml.WB: Preliminary experiments gave an approx 80kDa band in human brain lysates at 0.5 μg/ml, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 72kDda according to NP_060693. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti CHFR antibody, anti checkpoint with forkhead
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 55743

Immunohistochemical staining of human brain using CHFR antibody