CHFR Antibody

SKU: orb18717

Description

Goat polyclonal antibody to CHFR

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution range: ELISA: 1:64000, WB: 0.5ug/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded human brain (cerebellum) shows stained Purkinje cells. Recommended concentration, 2.5ug/ml.WB: Preliminary experiments gave an approx 80kDa band in human brain lysates at 0.5 μg/ml, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 72kDda according to NP_060693. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CHFR
• Molecular Weight: 73.4; 73.3; 72.0; 69.2; 63.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti CHFR antibody, anti checkpoint with forkhead and ring finger domains antibody, anti FLJ10796 antibody, anti FLJ33629 antibody, anti RNF116 antibody, anti RNF196 antibody, anti E3 ubiquitin-protein ligase CHFR antibody, anti RING finger protein 196 antibody

Immunohistochemical staining of human brain using CHFR antibody

Quick Database Links

Gene Symbol

CHFR

NCBI Gene ID

55743