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Goat anti-ADRB1 Antibody

Catalog Number: orb19192

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb19192
CategoryAntibodies
DescriptionGoat polyclonal antibody to beta 1 Adrenergic Receptor
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC
ReactivityCanine, Human, Mouse, Rat
Dilution rangeP-ELISA: 1:4000, IHC-P: 4-6μg/ml
ConjugationUnconjugated
MW51.2
TargetADRB1
Entrez153
Protein SequenceESDEARRCYNDPK
RRIDAB_10755903
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti-ADR B1 antibody, anti-ADRB 1 antibody, anti-A
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 55kDa band observed in Human Heart lysates (calculated MW of 51.2kDa according to NP_000675.1). Recommended concentration: 0.3-1 μg/ml.Experiment Notes: This product has been successfully used in assay standardization (beating frequency) of rat cardiomyocytes (PubMed: Wallkut G. et al., Am Coll Cardiol. 2010 Feb 2;55(5):463-8.; PMID: 20117461). Recommended concentration: 5ug/ml.
Expiration Date12 months from date of receipt.
Goat anti-ADRB1 Antibody

orb19192 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining.

Goat anti-ADRB1 Antibody

orb19192 Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.

Goat anti-ADRB1 Antibody

orb19192 (6µg/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Goat anti-ADRB1 Antibody

Immunohistochemical staining of Human Heart using beta 1 Adrenergic Receptor antibody.

Goat anti-ADRB1 Antibody

Immunohistochemical staining of Human Heart using beta 1 Adrenergic Receptor antibody.