Goat anti-ADRB1 Antibody

• Catalog Number: orb19192
• Category: Antibodies
• Description: Goat polyclonal antibody to beta 1 Adrenergic Receptor
• Target: ADRB1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Canine, Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: ESDEARRCYNDPK
• RRID: AB_10755903
• MW: 51.2
• Tested applications: ELISA, IF, IHC
• Dilution range: P-ELISA: 1:4000, IHC-P: 4-6μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 55kDa band observed in Human Heart lysates (calculated MW of 51.2kDa according to NP_000675.1). Recommended concentration: 0.3-1 μg/ml.Experiment Notes: This product has been successfully used in assay standardization (beating frequency) of rat cardiomyocytes (PubMed: Wallkut G. et al., Am Coll Cardiol. 2010 Feb 2;55(5):463-8.; PMID: 20117461). Recommended concentration: 5ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-ADR B1 antibody, anti-ADRB 1 antibody, anti-A
• Note: For research use only
• Entrez: 153

Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.

4 µg/ml staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.