You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb19192 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to beta 1 Adrenergic Receptor |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC |
Reactivity | Canine, Human, Mouse, Rat |
Dilution range | P-ELISA: 1:4000, IHC-P: 4-6μg/ml |
Conjugation | Unconjugated |
MW | 51.2 |
Target | ADRB1 |
Entrez | 153 |
Protein Sequence | ESDEARRCYNDPK |
RRID | AB_10755903 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti-ADR B1 antibody, anti-ADRB 1 antibody, anti-A Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 55kDa band observed in Human Heart lysates (calculated MW of 51.2kDa according to NP_000675.1). Recommended concentration: 0.3-1 μg/ml.Experiment Notes: This product has been successfully used in assay standardization (beating frequency) of rat cardiomyocytes (PubMed: Wallkut G. et al., Am Coll Cardiol. 2010 Feb 2;55(5):463-8.; PMID: 20117461). Recommended concentration: 5ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb19192 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining.
orb19192 Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.
orb19192 (6µg/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemical staining of Human Heart using beta 1 Adrenergic Receptor antibody.
Immunohistochemical staining of Human Heart using beta 1 Adrenergic Receptor antibody.