ADRB1 Rabbit Polyclonal Antibody

SKU: orb329873

Description

Rabbit polyclonal antibody to beta 1 Adrenergic Receptor

Images & Validation

• Tested Applications: WB
• Reactivity: Human
• Predicted Reactivity: Canine, Guinea pig, Mouse, Porcine, Rabbit, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ADRB1
• Target: ADRB1
• Molecular Weight: 51 kDa
• Purification: Affinity Purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

anti-ADR B1 antibody, anti-ADRB 1 antibody, anti-ADRB1 antibody, anti-ADRB1R antibody, anti-Adrenergic beta 1 receptor antibody, anti-Adrenoceptor beta 1 antibody, anti-B1AR antibody, anti-beta 1 adrenoceptor antibody, anti-beta 1 adrenoreceptor antibody, anti-beta-1 adrenergic receptor antibody, anti-beta-1 adrenoceptor antibody, anti-beta-1 adrenoreceptor antibody, anti-beta1AR antibody, anti-RHR antibody

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

Sample Tissue: Human Jurkat Whole Cell, Antibody Dilution: 1 ug/mL.

Sample Type: Human Adult Placenta, Antibody Dilution: 1.0 ug/mL.

Sample Type: Human Fetal Heart, Antibody Dilution: 1.0 ug/mL.

Sample Type: Human Fetal Lung, Antibody Dilution: 1.0 ug/mL.

Sample Type: Human Fetal Muscle, Antibody Dilution: 1.0 ug/mL.

Positive control (+): Mouse liver (M-LI), Negative control (-): Mouse spleen (M-SP), Antibody concentration: 1 ug/mL.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 0.5, 5, and 50 ug/mL. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for either two or three concentrations were averaged and results and standard deviation are shown.

WB Suggested Anti-ADRB1 Antibody, Positive Control: Lane 1: 20 ug Wild type mouse, left ventricle Lane 2: 20 ug Transgenic mouse, treated with experimental drug, left ventricle Lane 3: 20 ug HepG2 lysate, Primary Antibody Dilution: 1:1000, Secondary Antibody: Goat anti rabbit-HRP, Secondry Antibody Dilution: 1:5000.

WB Suggested Anti-ADRB1 Antibody Titration: 0.2-1 ug/mL, ELISA Titer: 1:312500, Positive Control: Human brain.

Quick Database Links

Gene Symbol

ADRB1

UniProt

P08588

RefSeq (Protein):NP_000675