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ADRB1 Rabbit Polyclonal Antibody

Catalog Number: orb501003

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb501003
CategoryAntibodies
DescriptionADRB1 Rabbit Polyclonal Antibody
TargetADRB1
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Predicted ReactivityCanine, Porcine
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human ADRB1 (181-250/477aa)
UniProt IDP08588
MW52 kDa
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesbeta 1 Adrenergic Receptor; beta 1 Adrenergic Rece
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NoteFor research use only
ADRB1 Rabbit Polyclonal Antibody

Blank control: U937. Primary Antibody (green line): Rabbit Anti-ADRB1 antibody (orb501003), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

ADRB1 Rabbit Polyclonal Antibody

Blank control: U937. Primary Antibody (green line): Rabbit Anti-antibody, dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells wereincubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

ADRB1 Rabbit Polyclonal Antibody

Sample: Heart (Mouse) Lysate at 30 ug, Heart (Rat) Lysate at 30 ug, Primary: Anti-ADRB1 (orb501003) at 1/200 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/3000 dilution, Predicted band size: 52 kD, Observed band size: 55 kD.

ADRB1 Rabbit Polyclonal Antibody

Tissue/Cell: mouse kidney cell, Incubation: Red line: Anti-ADRB1 Polyclonal Antibody, FITC conjugated 1:200, 40 minutes at 37°C. Green line: Isotype Control (rabbit IgG/FITC) 1:200, 40 minutes at 37°C.

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    Canine, Guinea pig, Mouse, Porcine, Rabbit, Rat

    Human

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    Polyclonal

    Unconjugated

    100 μl
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    FC,  IF,  IHC-Fr,  IHC-P,  WB

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    Unconjugated

    50 μl, 100 μl, 200 μl
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    200 μl, 100 μl, 30 μl
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    ELISA,  IF,  IHC-P,  WB

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    FC,  IF

    Canine, Porcine

    Human, Mouse, Rat

    Rabbit

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    100 μl