ADRB1 Rabbit Polyclonal Antibody

SKU: orb501003

Description

ADRB1 Rabbit Polyclonal Antibody

Research Area

Adrenergic Receptors, Cardiovascular Research, Cell Biology, G Protein-Coupled Receptors, GPCR Signaling Pathway

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ADRB1 (181-250/477aa)
• Target: ADRB1
• Molecular Weight: 52 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

beta 1 Adrenergic Receptor; beta 1 Adrenergic Receptor; ADRB1R; Adrenergic beta 1 receptor; B1AR; beta 1 adrenoceptor; beta 1 adrenoreceptor; beta-1 adrenergic receptor; beta-1 adrenoceptor; beta-1 adrenoreceptor; BETA1AR; RHR; ADR B1; ADRB 1; ADRB1; ADRB1_HUMAN; ADRB1R; Adrenergic beta 1 receptor; Adrenoceptor beta 1.

Blank control: U937. Primary Antibody (green line): Rabbit Anti-ADRB1 antibody (orb501003), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: U937. Primary Antibody (green line): Rabbit Anti-antibody, dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells wereincubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Heart (Mouse) Lysate at 30 ug, Heart (Rat) Lysate at 30 ug, Primary: Anti-ADRB1 (orb501003) at 1/200 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/3000 dilution, Predicted band size: 52 kD, Observed band size: 55 kD.

Tissue/Cell: mouse kidney cell, Incubation: Red line: Anti-ADRB1 Polyclonal Antibody, FITC conjugated 1:200, 40 minutes at 37°C. Green line: Isotype Control (rabbit IgG/FITC) 1:200, 40 minutes at 37°C.

Quick Database Links

Gene Symbol

ADRB1

UniProt

P08588