Glutamine Synthetase antibody

SKU: orb2276276

Description

Glutamine Synthetase antibody validated for immunohistochemistry on 76 different Normal Tissues

Research Area

Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Liver: A strong GS staining should be seen of centrilobular hepatocytes while staining is weak to moderate in Kupffer cells. Negative Control: Liver: Periportal hepatocytes must be GS negative in the normal liver. Cellular Localization: Cytoplasm. Mitochondrion Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

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Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2c, kappa
• Clone No.: MSVA-750M
• Immunogen: Recombinant fragment (around aa 50-250) of human GLUL protein (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cell proliferation-inducing protein 59; GLUL; Glutamate ammonia ligase; GS; PIG43; PIG59; Glutamate decarboxylase; glutamine synthase; Glutamine synthetase; glutamine synthetase I; Proliferation inducing protein 43; GLNA; GLNS

Quick Database Links

UniProt

P15104