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ASNS Rabbit Polyclonal Antibody

Catalog Number: orb499801

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb499801
CategoryAntibodies
DescriptionASNS Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Canine, Equine, Porcine, Rabbit
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human ASNS (1-100/561aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:1000-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
TargetASNS
UniProt IDP08243
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesAsparagine synthetase; ASNS_HUMAN; Asparagine synt
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NoteFor research use only
Expiration Date12 months from date of receipt.
ASNS Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ASNS) Polyclonal Antibody, Unconjugated (orb499801) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

ASNS Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ASNS) Polyclonal Antibody, Unconjugated (orb499801) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

ASNS Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ASNS) Polyclonal Antibody, Unconjugated (orb499801) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

ASNS Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ASNS) Polyclonal Antibody, Unconjugated (orb499801) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

ASNS Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ASNS) Polyclonal Antibody, Unconjugated (orb499801) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

ASNS Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Asparagine synthetase) Polyclonal Antibody, Unconjugated (orb499801) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

ASNS Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ASNS) Polyclonal Antibody, Unconjugated (orb499801) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

ASNS Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ASNS) Polyclonal Antibody, Unconjugated (orb499801) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

ASNS Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Spleen tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Mouse Cerebellum tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Lane 5: Rat Cerebellum tissue lysates, Lane 6: Human HeLa cell lysates, Lane 7: Human Jurkat cell lysates, Lane 8: Human SH-SY5Y cell lysates, Lane 9: Human U87MG cell lysates, Primary: Anti-ASNS (orb499801) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 64 kDa, Observed band size: 60 kDa.

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