GLUL Antibody (N-term)

SKU: orb37151

Description

Peptide affinity purified rabbit polyclonal antibody.

Research Area

Cell Biology, Neuroscience

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:10-50
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB21070
• Immunogen: Synthetic Peptide
• Target: GLUL
• Molecular Weight: 42064
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Glutamine synthetase antibody, anti-GS antibody, anti-Glutamate--ammonia ligase antibody, anti-GLNS antibody, anti-GLUL antibody

Anti-GLUL Antibody (N-term) at 1:1000 dilution + Raji whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from Hela, Raji, Jurkat, HT-29 cell line (from left to right), using GLUL Antibody (N-term). Diluted at 1:2000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

All lanes: Anti-GLUL Antibody (N-term) at 1:2000 dilution. Lane 1: mouse cerebellum lysates. Lane 2: Jurkat whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-GLUL Antibody (N-term) at 1:2000 dilution. Lane 1: Jurkat whole cell lysates. Lane 2: mouse cerebellum lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from Hela, HT-29, Jurkat cell line, mouse brain, mouse cerebellum, rat cerebellum tissue lysate (from left to right), using GLUL Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

All lanes: Anti-GLUL Antibody (N-term) at 1:2000 dilution. Lane 1: mouse brain lysates. Lane 2: Hela whole cell lysates. Lane 3: Jurkat whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-GLUL Antibody (N-term) at 1:2000 dilution. Lane 1: HT-29 whole cell lysates. Lane 2: mouse cerebellum lysates. Lane 3: Raji whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

GLUL

UniProt

P15104

RefSeq (Protein):NP_001028228.1, NP_002056.2, NP_001028216.1