GLUT1 Antibody / SLC2A1

SKU: orb606644

Description

Recognizes a protein of 45-55kDa, which is identified as GLUT1. Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are many types of glucose transport carrier proteins, designated as Glut1 to Glut12. Glut1 is a major glucose transporter in the mammalian blood-brain barrier. It is expressed in high density on the membranes of human erythrocytes and the brain capillaries that comprise the blood-brain barrier. Glut1 is expressed at variable levels in many human tissues. Overexpression has been linked to tumor progression or poor survival of patients with carcinomas of the colon, breast, cervical, lung, bladder and mesothelioma. Glut1 is a sensitive and specific marker for the differentiation of malignant mesothelioma (positive) from reactive mesothelium (negative).

Images & Validation

• Tested Applications: ELISA, FACS, IF, IHC-P, WB
• Dilution range: ELISA: 1-2ug/ml for coating (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the GLUT1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: GLUT1/2476
• Immunogen: A portion of amino acids 203-305 from the human protein was used as the immunogen for this GLUT1 antibody.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Flow cytometry testing of human K562 cells with GLUT1 antibody (clone GLUT1/2476); Red = isotype control, Blue = GLUT1 antibody.

IHC testing of human breast carcinoma with GLUT1 antibody (clone GLUT1/2476). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of human K562 cells with GUT1 antibody (green, clone GLUT1/2476) and Reddot nuclear stain (red).

Western blot testing of human A431 cells with GLUT1 antibody (clone GLUT1/2476). Expected molecular weight: 45-55 kDa.

IHC testing of human bladder with GLUT1 antibody (clone GLUT1/2476). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using GLUT1 antibody (clone GLUT1/2476). These results demonstrate the foremost specificity of the GLUT1/2476 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free GLUT1 antibody (clone GLUT1/2476) as confirmation of integrity and purity.

Quick Database Links

UniProt

P11166