GLUT1 Rabbit Polyclonal Antibody

• Catalog Number: orb10725
• Category: Antibodies
• Description: GLUT1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GLUT1 (191-270/492aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54 kDa
• Target: SLC2A1
• UniProt ID: P11166
• RRID: AB_10751661
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Glucose Transporter GLUT1; GT-1; GLUT-1; GLUT 1; S
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Srijon K Banerjee 1, Lance R Thurlow 2, Kartik Kannan 1, Anthony R Richardson Glucose transporter 1 is essential for the resolution of methicillin-resistant S. aureus skin and soft tissue infections Cell Rep, (2024)

PubMed: 38990718

Reactivity

Mouse

Mouse splenocytes stained with Rabbit Anti-GLUT1 Polyclonal Antibody, PE conjugated at 1:50.

Sample: Lane 1: Heart (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Muscle (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-GLUT1 (orb10725) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 52 kD.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GLUT1 Polyclonal Antibody, Unconjugated (orb10725) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.