GLUT1 Rabbit Polyclonal Antibody

SKU: orb10725

Description

GLUT1 Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cardiovascular Research, Diabetes Research, Metabolism

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GLUT1 (191-270/492aa)
• Target: SLC2A1
• Molecular Weight: 54 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Glucose Transporter GLUT1; GT-1; GLUT-1; GLUT 1; Solute carrier family 2; facilitated glucose transporter member 1; Glucose transporter type 1; erythrocyte/brain; DYT17; DYT18; Erythrocyte/brain HepG2 glucose transporter; Erythrocyte/hepatoma glucose transporter; Glucose transporter 1; Glucose transporter type 1; Glucose transporter type 1 erythrocyte/brain; Glucose transporter type 1, erythrocyte/brain; GLUT; GLUT1; GLUT1DS; GLUTB; GT1; GTG1; Gtg3; GTR1_HUMAN; HepG2 glucose transporter; MGC141895; MGC141896; PED; RATGTG1; SLC2A 1; SLC2A1; Solute carrier family 2(facilitated glucose transporter), member 1; Solute carrier family 2 facilitated glucose transporter member 1.

Mouse splenocytes stained with Rabbit Anti-GLUT1 Polyclonal Antibody, PE conjugated at 1:50.

Sample: Lane 1: Heart (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Muscle (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-GLUT1 (orb10725) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 52 kD.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GLUT1 Polyclonal Antibody, Unconjugated (orb10725) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

SLC2A1

UniProt

P11166

References

Srijon K Banerjee 1, Lance R Thurlow 2, Kartik Kannan 1, Anthony R Richardson Glucose transporter 1 is essential for the resolution of methicillin-resistant S. aureus skin and soft tissue infections Cell Rep, (2024)

PubMed: 38990718

Reactivity

Mouse