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GLRA1/Glycine Receptor Alpha 1 Antibody

SKU: orb1535111

Description

GLRA1/Glycine Receptor Alpha 1 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P, WB
Dilution RangeIHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.3 - 1 µg/ml)
ReactivityBovine, Canine, Equine, Gallus, Hamster, Human, Mouse, Porcine, Rabbit
Application Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 60kD band observed in Mouse and Rat Testis lysates (calculated MW of 51.7kD according to Mouse NP_065238.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 ug/ml.

Key Properties

HostGoat
ClonalityPolyclonal
ImmunogenPeptide with sequence CLQAKDGISVKGAN, from the internal region of the protein sequence according to NP_037265.1.
TargetGLRA1/Glycine Receptor Alpha 1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration0.5 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

GLRA1, Alpha 1 glycine Receptor, GlyR alpha 1, GLYRA1, SPD, STHE, Spasmodic, Glycine receptor alpha 1, Glycine receptor, alpha 1, HKPX1, Strychnine binding subunit

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GLRA1/Glycine Receptor Alpha 1 Antibody

GLRA1 antibody (0.3 ug/ml) staining of Mouse Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

GLRA1/Glycine Receptor Alpha 1 Antibody (orb1535111)

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50 μg
$ 670.00
DispatchUsually dispatched within 2-3 weeks
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