GlyR alpha 1 + 2 + 3 Rabbit Polyclonal Antibody

• Catalog Number: orb10729
• Category: Antibodies
• Description: GlyR alpha 1 + 2 + 3 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GlyR alpha 1 (101-200/449aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 49 kDa
• Target: GLRA1
• UniProt ID: Q6DJV9
• RRID: AB_10747015
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: glycine receptor alpha 1; glycine receptor alpha 2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Mouse Testis tissue lysates, Lane 4: Mouse Lung tissue lysates, Lane 5: Rat Cerebrum tissue lysates, Lane 6: Rat Cerebellum tissue lysates, Lane 7: Rat Testis tissue lysates, Lane 8: Human HeLa cell lysates, Lane 9: Human A431 cell lysates, Lane 10: Human SH-SY5Y cell lysates, Primary: Anti-GlyR alpha 1 + 2 + 3 (orb10729) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kDa, Observed band size: 53 kDa.

Sample: Spinal cord (Mouse) Lysate at 40 ug, Spinal cord (Rat) Lysate at 40 ug, Primary: Anti-GlyR alpha 1 + 2 + 3 (orb10729) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kD, Observed band size: 54 kD.