Glycine receptor, alpha 1 Antibody

• Catalog Number: orb1248511
• Category: Antibodies
• Description: Glycine receptor, alpha 1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Porcine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: CLQAKDGISVKGAN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Mouse and Rat Testis lysates (calculated MW of 51.7kDa according to Mouse NP_065238.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Lung and Kidney. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 60kDa
• Target: Glycine receptor, alpha 1
• UniProt ID: P23415
• NCBI: NP_037265.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Glra1, glycine receptor, alpha 1, GLYRA1, glycine
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Mouse and Rat Testis lysates (calculated MW of 51.7kDa according to Mouse NP_065238.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Lung and Kidney. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248511 (0.3 ug/ml) staining of Mouse Testis lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248511 (5 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248511 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.