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Description
Images & Validation
−| Tested Applications | ELISA, FC, ICC, IHC, IP, WB |
|---|---|
| Dilution Range | Optimal antibody dilution should be determined by titration, however as a guideline try:WB: Use at an assay dependent dilution. Detects a band of approximately 105 kDa (predicted molecular weight: 99 kDa).Flow Cyt: Use 1µg for 106 cells.ICC/IF: 1/50. |
| Reactivity | Hamster, Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 12H12 |
| Immunogen | Recombinant 6His-tag COOH-terminal domain of Gemin3 (amino acids 368-548) |
| Target | Gemin 3 |
| Purification | Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody (from supernatant) containing PBS + 0.1% sodium az |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Component of gems 3; DDX 20; DEAD box protein; DEAD-box protein DP103; DEAD/H BOX 20; DKFZP434H052; DP103; SMN interacting protein;
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation















