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Description
Images & Validation
−| Tested Applications | ELISA, FC, ICC, IHC, IP, WB |
|---|---|
| Dilution Range | Optimal antibody dilution should be determined by titration, however as a guideline try:WB: Use at an assay dependent dilution. Detects a band of approximately 105 kDa (predicted molecular weight: 99 kDa).Flow Cyt: Use 1µg for 106 cells.ICC/IF: 1/50. |
| Reactivity | Hamster, Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 12H12 |
| Immunogen | Recombinant 6His-tag COOH-terminal domain of Gemin3 (amino acids 368-548) |
| Target | Gemin 3 |
| Purification | Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody (from supernatant) containing PBS 0.1% sodium azide; |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Component of gems 3 antibody;DDX 20 antibody;DDX20 antibody;DDX20_HUMAN antibody;DEAD (Asp Glu AlaAsp) box polypeptide 20 antibody;DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 antibody;DEAD box protein 20antibody;DEAD box protein DP 103 antibody;DEAD box protein DP103 antibody;DEAD-box protein DP103antibody;DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 103kD antibody;DEAD/H (Asp Glu Ala Asp/His) boxpolypeptide 20 antibody;DEAD/H BOX 20 antibody;DKFZP434H052 antibody;DP 103 antibody;DP103 antibody;Gemin-3antibody;Gemin3 antibody;Probable ATP dependent RNA helicase DDX20 antibody;Probable ATP-dependent RNA helicaseDDX20 antibody;SMN interacting protein antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation















