GATA3 antibody

SKU: orb2276434

Description

GATA3 antibody validated for immunohistochemistry on 76 different Normal Tissues

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:50-1:100
• Reactivity: Human
• Application Notes:
  Positive Control: Kidney: A moderate to strong nuclear staining reaction should be seen in a fraction of collecting duct cells and podocytes in glomeruli. ; Tonsil: The vast majority of T helper cells (Th2) in the T-zones should show a weak to moderate nuclear staining. Negative Control: Kidney: Staining should be absent in proximal and distal tubuli as well as in blood vessels. ; Tonsil: Staining should be absent in B-cells. Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:80 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit according to the manufacturer’s directions.

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Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone No.: MSVA-450M
• Immunogen: Recombinant full-length human GATA-3 protein
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GATA3; GATA binding protein-3; GATA-binding factor 3; GATA3; HDR; HDRS; Transacting T-cell-specific transcription factor GATA-3

Quick Database Links

UniProt

P23771